The adhesive mat frame is intended for use with peel off mats. The frame allows for easy relocation or removal for maintenance. Also it prevents adhesive from building up a residue on the cleanroom floor. The frame has a border around the frame, and is sized accordingly to available mat sized. It adheres to the floor with a web coating without leaving any residue or adhesive and can be used in clean environments. Adhesive mats can be removed and re-applied for re-use of the frame. Available in either Aluminum or Polysyrene Plasitc.